Analysis of transgenic and non-transgenic rabbit milk
2005
Chrenek, P.(Vyskumny ustav zivocisnej vyroby, Nitra (Slovak Republic) | Dragin, S. | Chrastinova, L. | Kirchnerova, K. | Rafay, J. | Bulla, J.
The aim of this preliminary study was to compare milk production and quality of transgenic and non-transgenic rabbit females on the I.- lactation. Transgenic animals (F0 generation) were made by embryotransfer of microinjected rabbit eggs with WAP-hFVIII gene construct into pronucleus of fertilized eggs. The next F1 generation was obtained after breeding of transgenic founder rabbits (F0 generation) with non-transgenic rabbit. Transgenic rabbits produced recombinant human factor VIII (rhFVIII) into milk. Possible effect on milk quality and quantity of transgenic females was compare with non-transgenic females. The amount of milk produced by does at 10, 15, 20 and 30 day of the I.- lactation by weight-suckle-weight method and milk quality (fat, protein, lactose, and number of somatic cells) of both group were measured. Comparison of milk production showed that there are no differences between transgenic and standard genotype females. Significant differences (P<0.05) in content of proteins, fat and number of somatic cells were received in transgenic rabbit milk comparing with non-transgenic one.
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