The using of molecular fingerprinting methods for identification and differentiation of maize hybrids (Zea mays L.)
2005
Hajkova, P.(Zemedelsky vyzkum, s.r.o., Troubsko (Czech Republic) | Porubova, M.CEZEA - slechtitelska stanice, a.s. Cejc (Czech Republic))
Modern breeding methods require new approaches to analysis of breeding material. The characterization and identification of plant material on molecular level is important part of molecular breeding development. The exact identification of maize hybrid lines is tool of autenticity and pedigree determination in possible commercial causes. The collection of 30 maize hybrids was analysed by means of the RAPD molecular method based on Polymerase Chain Reaction. The preliminary screening of the plant material was accomplished by the collection of 37 primers on the two- and three-lines maize hybrids. 14 primers for the analysis of 30 hybrids were selected on basis of detected polymorphism. After repeated analysing of this 14 primers were selected 4 primers, that in common can differentiate this collection of 30 maize hybrid lines. All hybrids were differentiated and the genetic relation between individual hybrids was determined in dependence on the used RAPD primers. The resulting RAPD profiles can be used for the exact identification of the analysed maize hybrid lines.
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