Using PCR multiplex method in identification and study of wheat technological quality
2005
Greganova, Z.(Slovenska polnohospodarska univ. v Nitre (Slovak Republic). Katedra biochemie a biotechnologie, Tr. A.Hlinku 2, 949 76 Nitra | Galova, Z. | Chnapek, M.
The aim of our work was to analyse a set of 33 new wheat genotypes and wheat cultivars for the presence of DNA marker which is linked with gene encoding HMW subunit 1DX5 associated with subunit 1Dy10. The allelic pair 1Dx5+1Dy10 has positive effect on wheat technological quality. A clear PCR product of 450 bp, representing 1DX5 of the Glu-D1 alleles, was identified in 26 new wheat genotypes and cultivars. PCR analysis of 1DX5 were compared with protein analysis done by SDS-PAGE. For the discrimination major HMW alleles at A, B and D genome loci was used a multiplexed PCR assay in a single PCR reaction and agarose gel assay. PCR analysis for gene controlling bread-making quality confirmed that primers used in the present study are specific for the studied alleles and can be used for the detection of the wheat technological quality.
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