The pollutants effect on nitrogen metabolism in in vivo and in vitro conditions
2005
Faixova, Z.(Univerzita veterinarskeho lekarstva, Kosice (Slovak Republic) | Faix, S.Slovenska akademia vied, Kosice (Slovak Republic). Ustav fyziologie hospodarskych zvierat | Makova, Z.Univerzita veterinarskeho lekarstva, Kosice (Slovak Republic) | Szaboova, R. | Vaczi, P. | Prosbova, M.
The aim of the study was to evaluate the effects of some pollutants on ALT, AST, GGT and GDH enzyme activities of ruminal fluid in vivo and in vitro conditions. In vivo experiment was carried out on ewes from an industrially exposed and control areas. The concentrations of Cu and Zn were significantly higher in the rumen fluid of sheep from the industrially exposed area. The activity of individual enzymes from industrially exposed area were significantly higher. In vitro experiment, Cu2+, Cd2+ and Zn2+ were examined to assess how individual enzyme activity of ruminal fluid might be affected. Cd was found to inhibit both GDH and GGT activity but on the other hand, it stimulated both ALT and AST activities. Cu inhibited GDH activity. Zn produced an activation of ALT, AST and GDH. Results of the study indicate that pollutants can affect ruminal enzyme activities of sheep and there are differences between the effect of pollutants added individually to ruminal fluid and effect of pollutants which are animals exposed to chronically in industrially exposed area. In vivo, resulting enzyme activity reflects mutual action of individual pollutants on enzyme system in the rumen environment.
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