Microsatellite markers of pm5 locus in Czech and Slovak wheat cultivars (Triticum aestivum L.)
2006
Civáň, P., Univerzita Komenského, Bratislava (Slovak Republic). Katedra genetiky | Švec, M., Univerzita Komenského, Bratislava (Slovak Republic). Katedra genetiky
Powdery mildew caused by Blumeria graminis DC. f. sp. tritici is one of the most important fungal diseases in common wheat (Triticum aestivum L.) worldwide. Recessive powdery mildew resistance gene pm5 seems to be multialelic and cultivars possessing this gene exhibit relatively high levels of resistance in field conditions, even though virulent races of pathogen have been stabilized in populations already. Slovak common wheat cultivar Ilona and Czech cultivars Regina and Zdar carry recessive powdery mildew resistance alleles pm5b, pm5a and unspecified pm5 allele, respectively. Two microsatellite markers, namely Xgwm1267 and Xgwm78S, closely linked to pm5 locus were used to distinguish different alleles in these cultivars. Highly polymorphic microsatellite marker Xgwm783 amplified various products in all tested cultivars and hence, it could be beneficial for marker-assisted selection and gene pyramiding in breeding programs. By means of bulked segregants, it was also demonstrated that new recessive powdery mildew resistance gene in Texas wheat variety TAM110 is not allelic with pm5 locus.
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