Arthrobotrys sp.: Biology, ecology and biological control potential in northern Thailand
2003
Pamorntip Aksorntong(Chiang Mai University, Chiang Mai (Thailand). Faculty of Agriculture. Department of Plant Pathology)
Numerous soil samples were collected from various crops grown plots located in the two main Royal Development Centre namely Khun Wang and Khae Noi under the Royal Project Foundation, Chiangmai Province. Nematophagous fungus was isolated by scattering 3ml soil onto water agar (WA) and biting the 9 cm diam. Petri-dish with 1 ml surface steriled nematode suspension. After 24 h, the hyphae grown unidirection from soil, within 48 h the nematode e.g. Rhabidis sp. and Meloidogyne javanica juveniles were trapped by adhesive network. Seventy-two hours later the mycelium fully grown inside the nematode body. Some network was observed to trap the nematode time and again. After inoculating nematodes for 3 days, conidiophore and conidia were observed and 11 isolates were considered resembling to Arthrobotrys oligospora Fresenius. Comparison of radial growth ability on different culture media by isolate KN01, which found less network traps, showed that media which soybean, mungbean, and brown rice used as ingredient mixed with dextrose agar provide good radial growth rates and showed equal hyphal density as compare to those cultured on corn meal agar (CMA).
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