Insecticide resistance of the green leafhopper, Nephotettix cincticeps, to systemic insecticides by parafilm test: a simple method to evaluate the efficiency of the insecticide
2003
Jirapong Jairin(Department of Agriculture, Bangkok (Thailand). Rice Research Institute. Ubon Ratchathani Rice Research Centre) | Kojima, Nobuki(Tsukuba International Centre, Ibaraki (Japan)) | Nagata, Toru(Ibaraki Unviersity, Ibaraki (japan). Faculty of Agriculture)
Insecticide susceptibility of the green leafhopper, Nephotettix cincticeps to systemic insecticides, imidacloprid, cartap, propaphos and disulfoton, were determined by a newly developed testing method, the parafilm test using an artificial membrane to give an artificial diet composed of 2.5 percent sucrose and series dilution of insecticides. This test can measure both mortality (LC sub(50)) and feeding inhibition (Fl sub(50)). When the three local populations of green leafhopper with different resistance levels to organophosphates and carbamates were compared with LC sub(50) and Fl sub(50) obtained by this testing method. Feeding inhibition at sublethal doses was found to be the most remarkable with imidacloprid, followed by cartap without regarding to resistance levels, while feeding inhibition by propaphos and disulfoton was observed only with the resistant green leafhopper.
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