Potential methods for identification the gamma irradiated from unirradiated larvae of Helicoverpa armigera Hubner (Lepidoptera: Noctuidae)
2004
Sermsiri Surisan(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agriculture. Department of Entomology) | Praparat Hormchan(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agriculture. Department of Entomology) | Surachate Jamornmarn(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agriculture. Department of Entomology) | Arunee Wongpiyasatid(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Science. Department of Applied Radiation and Isotopes) | Arunee Engkakul(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Science. Department of Biochemistry)
To verify the irradiated from unirradated Helicoverpa armigera larvae through the two methods, the 1st , 2nd , 3rd and 4th instar larvae were irradiated with gamma radiation at 0, 75 and 150 Gy and observed in the late 5th instar. Irradiated at 75 Gy, the total haemocyte counts (THCs) of the irradiated 1st, 2nd , 3rd and 4th were found to be 6207+-178, 6990+-215, 9014+-244 and 9991+-279 h/cubic mm respectively while those irradiated at 150 Gy averaged 3939+-156, 4988+-157, 5704+-203 and 5904+-218 h/cubic mm respectively. It was also revealed that phenoloxidase (PO) activities of the irradiated 1st, 2nd, 3rd and 4th larval stages were 2.6+-0.7, 9.7+-0.7, 16.6+-2.6 and 23.7+-2.6 units/mg protein respectively whereas those irradiated at 150 Gy averaged 0.8+-0.1, 4.6+-0.5 , 10.4+-1.3 and 15.0+-1.4 units/mg protein respectively. The results of the effects of radiation on THC and PO activity supported each other. The changes in such experiments on each instar at each dose were found to be significantly less than THC (12921+-605 h/cubic mm) and PO activity (31.6 units/mg protein) of the controls. In both doses, percent reduction of THC and PO activity also increased from the 4th to 1st instars.
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