Genetic diversity of Andrographis paniculata Wall. ex Nees as revealed by morphological characters and molecular markers
2005
Tathaporn Maison(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Center for Agricultural Biotechnology) | Volkaert, Hugo(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Center for Agricultural Biotechnology) | Unaroj Boonprakob(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture. Department of Horticulture) | Yingyong Paisooksantivatana(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agriculture. Department of Horticulture)
Intraspecific variation of twenty-five accessions of Andrographis paniculata Wall. ex Nees, collected from different geographical areas in Thailand and one from Laos, was determined by morphological characters and molecular analysis using RAPD and SSCP techniques. Morphological characters and total lactone content variation were measured at 50 percent flowering time among the accessions grown under uniform environmental condition. UPGMA cluster analysis of all accessions resulted in 4 major groups based on 18 morphological characters while RAPD analysis distinguished 5 groups at 0.82 of similarity coefficient, using 247 bands generated from 14 primer amplifications separated on polyacrylamide gel. Morphological characters and RAPD were incongruent and were not correlated to geographical area of collection and yield of active compound. SSCP analysis showed little polymorphism of specific amplified products. Markers for the CPS1-2, IDH1, IDH2 and IPI genes were monomorphic and only 2 alleles were detected for CAT and CPS1-1 genes.
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