Analysis of antimicrobial drug resistance in enteropathogenic Escherichia coli (EPEC) isolated in rabbits with diarrhoeic syndrome
2008
Urumova, V., Thracian University, Stara Zagora (Bulgaria) | Petrov, V., Thracian University, Stara Zagora (Bulgaria)
The sensitivity of 51 enteropathogenic Escherichia coli (EPEC) strains, isolated from recently weaned rabbits with diarrhoeic syndrome to 13 antimicrobial drugs has been tested. The samples were obtained from six farms located in North and South Bulgaria. The inhibition zones were interpreted by the three-degree system of Bauer-Kirby's disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI) NCCLS requirements. A phenotype analysis of resistance of isolated rabbit E. coli strains to the different groups of tested antimicrobial drugs was performed. The analysis included drawing of experimental cumulative curves and their theoretical analoques as well as determinations of the inhibition zone diameters in 50% and 90% of isolates, the so-called ZD50 and ZD90 correspondingly to MIC50 and MIC90. The resistance patterns in studied strains were determined. The highest incidence (15.6%) was that of patterns including resistance to amoxicillin, cefalotin and cefuroxime as the profile including resistance to beta-lactams and flu equine.
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