Cloning and sequencing analysis of a flowering-related MA DS-box Gene in bambusa oldhamii L.
2007
Gao Zhimin, Liu Yingli , Li Xueping
chinois. MADS―box基因编码的转录因子在植物花器官发育过程中起着重要作用。采用RT-PCR技术，从绿竹（Bambusa oldhamii L．）中分离到一个MADS―box基因，命名为BOMADS1（GenBank登记号：EF517293）。BOMADS1编码区cDNA全长723bp；编码区共编码240个氨基酸，具有典型的植物MADS-box基因结构，其编码肽链包含了MADS区、K区、I区和C末端。序列分析结果表明，BOMADS1编码的蛋白与其它植物的MADS―box蛋白有着较高的一致性，其中与石刁柏（Asparagus officinalis L．）的AOM3高达87％。系统进化树分析表明BDMADS1基因属于E类功能基因。
Afficher plus [+] Moins [-]anglais. Floral homeotic MADS-box genes encoding transcription factors play important roles in determining floral organ identities. The flowering-related MADS-box gene, named as BOMADS1 （GenBank accession number： EF517293）, was cloned from the first strand of Bambusa oldhamii L. cDNA through RT-PCR using primers designed according to the MADS-box gene family conserved region. The fragment was 723 bp length and contained an open reading frame coding a polypeptide of 240 amino acids which had a typical MADS-box gene structure including MADS-box domain, K domain, I region and C terminus. Homology analysis indicated that the deduced BOMADS1 protein was highly homologous to other MADS-box proteins from different species, especially with AOM3 from Asparagus officinalis up to 87%. Phylogenetic tree analysis also indicated that BOMA DS1 might belong to E function genes.
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par Institute of Agricultural Information, Chinese Academy of Agricultural Sciences
Découvrez la collection de ce fournisseur de données dans AGRIS