In vitro propagation of Capsicum chinense Jacq.
2008
Sanatombi, K.,Manipur Univ., Imphal (India). Dept. of Life Sciences | Sharma, G.J.,Manipur Univ., Imphal (India). Dept. of Life Sciences
An efficient micropropagation protocol was established for Capsicum chinense cv. Umorok, a pungent chilli cultivar. Shoot-tip explants were cultured on Murashige and Skoog medium containing cytokinins (22.2-88.8 microM 6-benzylaminopurine, BAP, 23.2-93.0 microM kinetin, Kin, or 22.8-91.2 microM zeatin, Z) alone or in combination with 5.7 microM indole-3-acetic acid. Maximum number of shoots were induced on medium containing 91.2 microM Z or 31.1 microM BAP with 4.7 microM Kin. The separated shoots rooted and elongated on medium containing 2.5 or 4.9 microM indole-3-butyric acid. Axillary shoots were induced from in vitro raised plantlets by decapitating them. The axillary shoot-tip explants were used for further multiple shoot buds induction. A maximum of about 150 plantlets were obtained from a single seedling. Hardened and acclimatized plantlets were successfully established in the soil.
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