An improved protocol for micropropagation of elite genotypes of Simmondsia chinensis (Link) Schneider
2008
Singh, A.,Central Salt and Marine Chemicals Research Inst., Bhavnagar (India). Discipline of Phytosalinity | Reddy, M.P.,Central Salt and Marine Chemicals Research Inst., Bhavnagar (India). Discipline of Phytosalinity | Patolia, J.S.,Central Salt and Marine Chemicals Research Inst., Bhavnagar (India). Discipline of Phytosalinity
An efficient micropropagation protocol was developed for elite male and female genotypes of Simmondsia chinensis using nodal segments. Bud initiation was found to be best on Murashige and Skoog's (MS) medium supplemented with 4.44 microM 6-benzylaminopurine (BAP) and 88.8 microM adenine. Upon sub-culture, 10-15 shoots per explant were obtained when 4.44 microM BAP and 74.0 microM adenine were incorporated in the medium. Increase in KNO3 concentration in the medium improved shoot multiplication rate and in vitro flowering in 20% of male cultures. Elongated shoots were harvested, pulse treated for 48 h on liquid medium supplemented with 49.0 microM indole-3-butyric acid, 5.40 microM alpha-naphthaleneacetic acid and 5.71 microM indole-3-acetic acid for root induction and rooting (92%) was achieved on hormonal free half-strength MS medium supplemented with 1.37 microM chlorogenic acid, 1% activated charcoal and 2% sucrose. After successful hardening, plantlets were transferred to greenhouse with 99% establishment.
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