Protein profiling in F1 and F2 generations of two tomato genotypes differing in ripening time
2008
Rodriguez, G.R.,Universidad Nacional de Rosario, Zavalla (Argentina). Catedra de Genetica | Sequin, L.,Instituto Nacional de Tecnologia Agropecuaria, Marcos Juarez (Argentina) | Pratta, G.R.,Universidad Nacional de Rosario, Zavalla (Argentina). Catedra de Genetica | Zorzoli, R.,Universidad Nacional de Rosario, Zavalla (Argentina). Catedra de Genetica | Picardi, L.A.,Universidad Nacional de Rosario, Zavalla (Argentina). Catedra de Genetica
Pericarp polypeptide profiles were analyzed at three ripening stages in the F1 hybrid and the F2 population from the cross between LA1385 (Lycopersicon esculentum var. cerasiforme) and 804627 (L. esculentum, a homozygous genotype for the nor mutant). Six polymorphic polypeptides were observed in LA1385, while no polymorphic polypeptides among ripening stages was observed in 804627. On the other hand, some polypeptides in the F1 hybrid were not observed in the parents, whereas others were present in both parental genotypes and were unnoticeable in the hybrid genotype. From a cluster analysis on the protein profiles of the F2 population, the differential expression of proteins allowed to distinguish mature green (MG) stage from the other two stages, while for breaker stage (BR) and red ripe stage, the genetic background was more important in forming groups. The differential expression of proteins could be associated with fruit morphology traits such as a 72 kDa polypeptide present in MG stage with fruit diameter, height and mass and a 47 kDa polypeptide found in BR with fruit shelf life.
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