Detection of DNA ladder during cotyledon senescence in cotton
2008
Xie, Q.E.,Shandong Agricultural Univ., Tai'an (China). State Key Lab. of Crop Biology | Liu, I.D.,Shandong Agricultural Univ., Tai'an (China). State Key Lab. of Crop Biology | Yu, S.X.,Chinese Academy of Agricultural Sciences, Anyang (China). Cotton Research Inst. | Wang, R.F.,Shandong Agricultural Univ., Tai'an (China). State Key Lab. of Crop Biology | Fan, Z.X.,Shandong Agricultural Univ., Tai'an (China). State Key Lab. of Crop Biology | Wang, Y.G.,Shandong Agricultural Univ., Tai'an (China). State Key Lab. of Crop Biology | Shen, F.F.,Shandong Agricultural Univ., Tai'an (China). State Key Lab. of Crop Biology
The asynchronous programmed cell death in different regions of the cotton cotyledon was studied during senescence. We showed that changes in chlorophyll contents do not occur at the same time in different parts of the cotyledon. By using light microscopy and the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end in situ labelling (TUNEL) methods, the symptoms of cell death were detected in cotyledon margins earlier than in the central part. DNA ladders were detected by gel electrophoresis in senescent cotyledon margins and in the centre respectively, but not in the whole cotyledons.
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