Determination of the content of rutin and total polyphenols in leaves of spinach and amaranth
2009
Martinkova, J.,Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Botaniky a Fyziologie Rostlin | Hnilicka, F.,Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Botaniky a Fyziologie Rostlin | Hnilickova, H.,Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Botaniky a Fyziologie Rostlin | Orsak, M.,Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Chemie
In the years 2002 to 2004, the concentrations of rutin and total polyphenols were determined in the leaves of spinach and amaranth using the HPLC method. The experiment included three amaranth species, love-lies-bleeding (A. caudatus), red-blooded amaranth (A. cruentus) and red-spiked amaranth (A. hypochondriacus), and two spinach varieties, Matador and Triptiek F1. The average rutin conc. was lowest at the beginning of vegetation (159.11 mg/g) and highest in the phase of stem elongation (294.10 mg/g). The highest average rutin conc. was recorded in A. hypochondriacus (234.54 mg/g) and the lowest one in A. cruentus (154.92 mg/g). The conc. of total polyphenols in spinach leaves was significantly influenced by the development. Their lowest average conc. was at the beginning of the spinach vegetation (10.95 mg/g) and the highest one in the 3rd sampling (formation of leaf rosette, 15.60 mg/g). Significant differences were found between the spinach varieties (14.29 mg/g in Triptiek F1 vs. 12.38 mg/g in Matador). The average conc. of polyphenols in the phase 24 was 10.38 mg/g, in the phase of full ripeness it was 15.40 mg/g. Polyphenols conc. was the lowest in A. caudatus (13.43 mg/g) and the highest one in A. hypochondriacus (15.24 mg/g). The studied characteristics were influenced by the year. In conclusion, leaves of amaranth and spinach should be harvested before the onset of anthesis.
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