Preliminary results of in vivo thermotherapy of plum, apricot and peach cultivars artificially infected with PPV-M and PPV-D strains of Plum pox virus
2009
Polak, J.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic) | Hauptmanova, A.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic)
The elimination of Plum pox virus (PPV) in different stone fruit cultivars was verified by the method of thermotherapy in vivo. Trees of two plum cultivars Cacanska lepotica and Svestka domaci, apricot cultivars Leskora and Velkopavlovicka, and peach cultivars Redhaven and Earliglo were used. They were infected artificially with two strains of the virus (PPV-D, PPV-M). Two cycles of thermotherapy in vivo were performed. During the first cycle, 16 trees of plum, apricot and peach were treated for 15 days at 37 deg C. In the second thermotherapy cycle, 10 trees of individual cultivars of plum, apricot and peach were treated for 22 days at 37 deg C. In the first thermotherapy (T1), 8 trees out of 16 died; PPV was eliminated in 2 trees of cv. Cacanska lepotica, 1 tree of cv. Svestka domaci and 2 trees of cv. Velkopavlovicka. In the second thermotherapy (T2), 1 of 10 treated trees died. The virus was eliminated in 2 trees of cv. Cacanska lepotica, 1 tree of cv. Leskora, 2 trees of cv. Velkopavlovicka, and 1 tree of cv. Redhaven. Nine (T1) and seven (T2) months after the thermotherapy, the presence of PPV was detected in 6 out of 11 originally recovered trees using ELISA. Four trees (2 plum trees and 2 apricot trees) out of 26 remained recovered. One of these trees, apricot cv. Leskora, was originally infected with PPV-M strain, whereas the other three trees were infected with PPV-D strain. None of the 10 peach trees was treated successfully.
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