Effect of freezing on biochemical constituents of sperm membrane extracts and seminal plasma of buffalo bulls
2002
Dhanju, C.K., Punjab Agricultural University, Ludhiana (India). Dept. of Zoology | Cheema, R.S., Punjab Agricultural University, Ludhiana (India). Dept. of Animal Breeding and Genetics | Kapur, S.P., Punjab Agricultural University, Ludhiana (India). Dept. of Zoology
The carbohydrates, lipids and cholesterol were estimated in both Deoxycholate (DOC) extract of spermatozoal membrane and seminal plasma of chilled as well as frozen semen of five Murrah buffalo bulls after 6, 12, 18 and 24 months of freezing. The carbohydrates, phospholipids and cholesterol content of sperm membrane extracts as well as of seminal plasma gradually decreased with increase in freezing time from 6-24 months, when compared with the amounts observed in freshly chilled spermatozoa in all the bulls except in two bulls in which the phospholipid content decreased up to six months and then there was a variable change later on. These observations thus revealed that freezing caused deleterious alterations in the plasma membrane constituents of the buffalo bull spermatozoa, which perhaps resulted in the decreased motility of course within permissible limits of fertility which was 5-10 percent in the present studies.
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