Low levels of Haptoglobin and putative amino acid sequence in Taiwanese Lanyu miniature pigs
2008
Yueh, S.C.H.(National Chiao Tung Univ. (Taiwan)) | Wang, Y.H. | Lin, K.Y. | Tseng C.F. | Chu, H.P. | Chen, K.J. | Wang, S.S. | Lai, I.H. | Mao, S.J.T.
Porcine haptoglobin (Hp) is an acute phase protein. Its plasma level increases significantly during inflammation and infection. One of the main functions of Hp is to bind free hemoglobin (Hb) and inhibit its oxidative activity. In the present report, we studied the Hp phenotype of Taiwanese Lanyu miniature pigs (TLY minipigs; n=3) and found their Hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human Hp 1-1. Interestingly, Western blot and high performance liquid chromatographic (HPLC) analysis showed that 25% of the TLY minipigs possessed low or no plasma Hp level (0.05 mg/ml). The Hp cDNA of these TLY minipigs was then cloned, and the translated amino acid sequence was analyzed. No sequences were found to be deficient; they showed a 99.7% identity with domestic pigs (NP-999165). The mean overall Hp level of the TLY minipigs (0.21+-0.25 mg/ml; n
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