Detection and characterization of radicals derived from 3-Deoxyglucosone in Maillard reaction
2008
Kobayashi, T.(Shimane Univ., Matsue (Japan). Faculty of Life and Environmental Science) | Yasuda, T. | Shibata, H.
3-Deoxyglucosone (3-DG), an alpha, beta-dicarbonyl compounds in phosphate buffer, pH 7.4 with amino acids, produced different ESR spectra depending on the added amino acids, by the use of 3, 5-dibromo-4-nitrosobenzenesulfonic acid (DBNBS) which is suitable for spin trapping of carbon centered radicals. 3-DG is known to polymerize protein, but added Lys promoted the dimerization of lysozyme by the involvement of radical generation. As the formation and accumulation of alpha, beta-dicarbonyl compounds is known as carbonyl stress, we proposed that these radicals derived from amino acid via Maillard reaction may contribute oxidative stress, main part of carbonyl stress.
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