Multiple bud formation by aseptically terminal floral apex culture of Sandersonia aurantiaca Hook
2008
Fujita, T.(Snow Brand Milk Products Co. Ltd., Kawagoe, Saitama (Japan). R and D Planning Dept.) | Kajikawa, Y. | Nishimura, A. | Fukui, H.
In order to establish efficient vegetative micropropagation of Sandersonia aurantiaca Hook., the effects of plant growth regulators on morphological changes of the terminal floral apex were investigated. All explants cultured on Murashige and Skoog medium supplemented with 0microM NAA and 3.2microM TDZ, 3.2microM NAA and 1microM TDZ, 3.2microM NAA and 3.2microM CPPU, or 3.2microM NAA and 3.2microM TDZ formed multiple buds. The multiple buds consisted of minute adventitious buds, and divided multiple buds proliferated by subculturing on the same component medium. Some multiple buds had elongated shoots, and a miniature tuber was formed at the base of the shoot. These results indicated that Sandersonia aurantiaca was rapidly micropropagated by terminal floral apex culture on four media.
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