Production of Raphanus sativus (C3)-Moricandia arvensis (C3-C4 intermediate) monosomic and disomic addition lines with each parental cytoplasmic background and their photorespiratory characteristics
2009
Bang, S.W.(Utsunomiya Univ. (Japan). Faculty of Agriculture) | Ueno, O. | Wada, Y. | Hong, S.K. | Kaneko, Y. | Matsuzawa, Y.
We are maintaining five Moricandia arvensis monosomic addition lines of Raphanus sativus carrying R. sativus cytoplasm (autoplasmic MALs) and twelve M. arvensis MALs of R. sativus carrying M. arvensis cytoplasm (alloplasmic MALs) from BCsub(6) to BCsub(8) generation, and newly produced five M. arvensis disomic addition lines of R. sativus (autoplasmic DALs) and seven M. arvensis DALs of R. sativus carrying M. arvenesis cytoplasm (alloplasmic DALs) from selfing and sib-crossing of the MALs and DALs in Ssub(3)BCsub(5) and Ssub(2)BCsub(6) generations. The structural, biochemical and physiological characteristics related to photorespiration of these MALs and DALs were compared to study the genetic mechanisms of the Csub(3)-Csub(4) intermediate photosynthesis in the individual chromosomes of M. arvensis. The CO2 compensation point of the autoplasmic and alloplasmic DALs (RMa-b and MaR-b DALs) with one pair of M. arvensis 'b' chromosome were 29.4 and 30.1 micromol/mol, respectively, which were significantly lower than that of other DALs and MALs as well as R. sativus (34.5 micromol/mol). An immunogold electron microscopic study of the P-protein of glycine decarboxylase (GDC) in photosynthetic cells of the RMa-b DAL revealed that the bundle sheath cell (BSC) mitochondria were more intensively labeled for the protein than the mesophyll cell (MC) mitochondria. The ratio of the labeling density of the BSC mitochondria to that of the MC mitochondria was 1.13, which lies between values in M. arvensis (2.66) and R. sativus (0.76). These data suggest that the 'b' chromosome of M. arvensis genome controls the expression of Csub(3)-Csub(4) intermediate characteristics.
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