Analysis of sugar composition in strawberry [Fragaria ananassa] using capillary electrophoresis
2009
Sato, K.(Fukuoka-ken. Agricultural Research Center, Chikushino (Japan)) | Horie, H. | Kitajima, N.
A protocol for washing capillary tubes was examined in connection with the analysis of sugars of strawberry using capillary electrophoresis. Methanol and 1M sodium hydroxide were found to be suitable for washing the capillary tube in the analysis of sugars. As a result of analyzing sugars using capillary electrophoresis with the improved washing method, 3 kinds of sugar in strawberry were separated. Coefficient of variation of detection time in 8 times continuous analysis was less than 1.1%. When sugars were extracted from strawberry fruits with water, heat-treatment with a microwave oven made sugar-metabolic enzymes inactive, and the changes in sugar composition were controlled. As a result of analyzing sugar composition of 3 strawberry cultivars using capillary electrophoresis, the differences between strawberry cultivars of sugar content and sucrose ratio (sucrose/sugar content) appeared to be consistent with past reports. It is hoped that the capillary electrophoresis described will be used to compare eating quality and sugar composition among strawberry cultivars as an inexpensive and simple method of sugar analysis.
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