Paternity testing of wild black rockfish Sebastes inermis (Brownish type) from the Seto Inland Sea of Japan
2009
Gonzalez, E.B.(Hiroshima Univ., Higashi-hiroshima (Japan)) | Murakami, T. | Teshima, Y. | Yoshioka, K. | Jeong, D.S. | Umino, T.
We developed four microsatellite DNA loci to test for multiple paternity of black rockfish, Sebastes inermis, from the Seto Inland Sea of Japan. All loci showed a high degree of polymorphism (number of alleles per locus = 10 to 14, expected heterozygosity = 0.80) and discriminating power (probability of identity index = 3.71 x 10E-6, exclusion probability = 0.999) in unrelated wild specimens (n = 32). Genotypic assignment of five dams (109-220 mm in total length) and 50 embryos from each dam (n = 50) indicated that four dams were mated with a single sire. Only for one dam and three of her embryos we could not exclude multiple paternity.
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