Cardiac Cell Differentiation of Mouse Embryonic Stem Cells in the Co-culture with Fetal Heart
2007
Park, K.M. (Bio-evaluation Center, Korea Research Institute of Biosciences and Biotechnology, Cheongwon, Republic of Korea) | Kim, E.K. (Bio-evaluation Center, Korea Research Institute of Biosciences and Biotechnology, Cheongwon, Republic of Korea) | Kim, H.L. (Bio-evaluation Center, Korea Research Institute of Biosciences and Biotechnology, Cheongwon, Republic of Korea) | Moon, O.S. (Bio-evaluation Center, Korea Research Institute of Biosciences and Biotechnology, Cheongwon, Republic of Korea) | Won, Y.S. (Bio-evaluation Center, Korea Research Institute of Biosciences and Biotechnology, Cheongwon, Republic of Korea) | Kim, H.C. (Bio-evaluation Center, Korea Research Institute of Biosciences and Biotechnology, Cheongwon, Republic of Korea) | Ahn, B.W. (Chungbuk National University, Cheongju, Republic of Korea) | Han, S.H. (The Catholic University of Korea, Seoul, Republic of Korea) | Nam, K.H. (Bio-evaluation Center, Korea Research Institute of Biosciences and Biotechnology, Cheongwon, Republic of Korea), E-mail: [email protected]
To investigate the effect of fetal cardiac cells on the differentiation of embryonic stem cells (ESCs) into cardiomyocytes, Mouse ESCs were co-cultured with heart cells prepared from fetus aged 11.5, 12.5, 13.5 or 14.5 days postcoitum (dpc). The fetal mouse heart was from ICR and 129 strains from which KH2 ES cell line was derived. In the present study, KH2 cells expressing EGFP directed by α-myosin heavy chain gene promoter were co-cultured with fetal heart isolated at different fetal stages.
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