Influences of LH, FSH, EGF and Cysteine on In Vitro Canine Oocyte Maturation
2007
Song, H.J. (Gyeongsang National University, Jinju, Republic of Korea) | Kang, E.J. (Gyeongsang National University, Jinju, Republic of Korea) | Ock, S.A. (Gyeongsang National University, Jinju, Republic of Korea) | Jeon, B.G. (Gyeongsang National University, Jinju, Republic of Korea) | Rho, G.J. (Gyeongsang National University, Jinju, Republic of Korea) | Choe, S.Y. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: [email protected]
Despite many efforts to improving canine in vitro maturation(IVM), the efficiency is still low compared to that of other mammalian species. The present study investigated the effects of gonadotropin, epidermal growth factor and cysteine supplementation on in vitro maturation of canine oocytes. Cumulus-oocyte complexes (COCs) were matured in basic medium (TCM-199 containing 10% FBS, 0.11 mg/ml sodium pyruvate supplemented with or without 10 ㎍/ml LH, 10 ㎍/ml FSH, 10 ng/ml EGF and 0.57 mM cysteine) for 72 hr at 38.5℃ in humidified atmosphere of 5% CO₂ in air. After culture, oocytes were stained with Hoechst 33342 (10 ㎍/ml) for 30 min at 4℃, and assessed their nuclear status (GV: germinal vesicle, GVBD: germinal vesicle break down, MI: metaphasse I, MII: metaphase II, UK: unknown stage). No differences were observed in GV, MI and MII rate except GVBD rate between with and without gonadotropins addition respectively (6.7% vs. 17.2%). Supplementation of 10 ng/ml of EGF in hormones added IVM medium resulted in significantly (p less than 0.05) higher MII rate (4.54% vs. 7.06%).
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