Optimal Culture Conditions for the Production of a Novel Extracellular Alkaline Lipase from Yarrowia lipolytica NRRL Y-2178
2007
Lee, G.H. (Kyungpook National University, Daegu, Republic of Korea) | Bae, J.H. (Kyungpook National University, Daegu, Republic of Korea) | Suh, M.J. (Kyungpook National University, Daegu, Republic of Korea) | Kim, H.R. (Kyungpook National University, Daegu, Republic of Korea), E-mail: [email protected]
Lipases are industrially useful versatile enzymes that catalyze numerous different reactions. Among lipases functioning under extreme conditions, alkaline lipase is useful in detergent industry. Lipase from yeast strain Yarrowia lipolytica NRRL Y-2178 was most active under alkaline condition, and initial medium pH for most lipase production was also alkaline [Lee et al., 2007, J Microbiol Biotechnol, 17(6)]. High lipase production was achieved using Y. lipolytica NRRL Y-2178. Optimal incubation time for lipase production at 25℃ was 72 h. Optimal temperature, when incubated for 72 h, was 27.5℃. Lipase production but not cell growth was very sensitive to concentrations of glucose and glycerol as efficient carbon sources, showing optimal concentrations of 1.0 and 1.5% (w/v), respectively. Lipase production was highly stimulated by Ca²+, K+, and Na+, but was inhibited by Co²+, Cu²+, Mn²+, Na+, and Fe²+. Maximum lipase production at 0.1 mM Ca²+ for 72 h incubation at 27.5℃ was 649 units/mL.
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