Detection of gene flow from GM to non-GM watermelon in a field trial
2008
Kim, C.G. (Bio-Evaluation Center, KRIBB, Cheongwon, Republic of Korea) | Lee, B.K. (Bio-Evaluation Center, KRIBB, Cheongwon, Republic of Korea) | Kim, D.I. (Bio-Evaluation Center, KRIBB, Cheongwon, Republic of Korea) | Park, J.E. (Bio-Evaluation Center, KRIBB, Cheongwon, Republic of Korea) | Kim, H.J. (Bio-Evaluation Center, KRIBB, Cheongwon, Republic of Korea) | Park, K.W. (Bio-Evaluation Center, KRIBB, Cheongwon, Republic of Korea) | Yi, H.B. (Bio-Evaluation Center, KRIBB, Cheongwon, Republic of Korea) | Jeong, S.C. (Bio-Evaluation Center, KRIBB, Cheongwon, Republic of Korea) | Yoon, W.K. (Bio-Evaluation Center, KRIBB, Cheongwon, Republic of Korea) | Harn, C.H. (Nongwoo Bio Co., Ltd., Yeoju, Republic of Korea) | Kim, H.M. (Bio-Evaluation Center, KRIBB, Cheongwon, Republic of Korea), E-mail: Hwanmook@kribb.re.kr
Gene flow from genetically modified (GM) crops to conventional non-GM crops is a serious concern for protection of conventional and organic farming. Gene flow from GM watermelon developed for rootstock use, containing cucumber green mottle mosaic virus (CGMMV)-coat protein (CP) gene, to a non-GM isogenic control variety 'Chalteok' and grafted watermelon 'Keumcheon' was investigated in a small scale field trial as a pilot study. Hybrids between GM and non-GM watermelons were screened from 1304 'Chalteok' seeds and 856 'Keumcheon' seeds using the duplex PCR method targeting the CGMMV-CP gene as a marker. Hybrids were found in all pollen recipient plots. The gene flow frequencies were greater for 'Chalteok' than for 'Keumcheon'; with 75% outcrossing in the 'Chalteok' plot at the closest distance (0.8 m) to the GM plot. A much larger scale field trial is necessary to identify the isolation distance between GM and non-GM watermelon, as the behaviors of insect pollinators needs to be clarified in Korea.
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