Chitosan oligosaccharides inhibit adipogenesis in 3T3-L1 adipocytes
2008
Cho, E.J. (Daegu University, Gyeongsan, Republic of Korea) | Rahman, Atiar (Daegu University, Gyeongsan, Republic of Korea) | Kim, S.W. (Daegu University, Gyeongsan, Republic of Korea) | Baek, Y.M. (Daegu University, Gyeongsan, Republic of Korea) | Hwang, H.J. (Daegu University, Gyeongsan, Republic of Korea) | Oh, J.Y. (Daegu University, Gyeongsan, Republic of Korea) | Hwang, H.S. (Daegu University, Gyeongsan, Republic of Korea) | Lee, S.H. (Daegu University, Gyeongsan, Republic of Korea) | Yun, J.W. (Daegu University, Gyeongsan, Republic of Korea), E-mail: jwyun@daegu.ac.kr
The 3T3-L1 cell line is a well-established and commonly used in vitro model to assess adipocyte differentiation. Over the course of several days, confluent 3T3-L1 cells can be converted to adipocytes in the presence of an adipogenic cocktail. In this study, the effects of chitosan oligosaccharides (CO) on adipocyte differentiation of 3T3-L1 cells were studied. The CO significantly decreased lipid accumulation, a marker of adipogenesis, in a dose-dependent manner. The low molecular mass CO (1-3 kDa) were the most effective at inhibiting adipocyte differentiation. Moreover, mRNA expression levels of both CCAAT/enhancer-binding protein (C/EBP) α and peroxisome proliferator-activated receptor (PPAR)γ, the key adipogenic transcription factors, were markedly decreased by CO treatments. CO also significantly downregulated adipogenic marker proteins such as leptin, adiponectin, and resistin. Our results suggest a role for CO as anti-obesity agents by inhibiting adipocyte differentiation mediated through the downregulated expression of adipogenic transcription factors and other specific genes.
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