Proline accumulation and growth of soybean callus under salt and water stress
2008
Kaviani, B. (Islamic Azad Univ., Rasht (Iran))
Calli of soybean [Glycine max cv. Pershing] were grown on "MS" media supplemented with different levels of KCl and NaH2PO4 for 45 days. The effects of salt stress were evaluated on the production and accumulation of proline and growth content of calli. Proline content increased as growth occurred. The maximum amount of proline and the highest content of growth were observed in calli grown on media supplemented with 60 mM KCl along with 1 mM NaH2PO4 (the highest salts levels) and 45 mM KCl along with 1 mM NaH2PO4, respectively. Proline accumulation was correlated with absorption of water from environment by calli that were grown on media containing 60 mM KCl and 1 mM NaH2PO4. The results suggest a positive role for proline accumulation in adaptation of cell to salt and water stress.
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