Some factors affecting the in vitro culture of banana
2006
Ahmad, T. (Pakistan Council of Scientific and Industrial Research Labs. Complex, Lahore (Pakistan). Food and Biotechnology Research Centre) | Zaidi, N. (Pakistan Council of Scientific and Industrial Research Labs. Complex, Lahore (Pakistan). Food and Biotechnology Research Centre) | Khan, N.H. (Pakistan Council of Scientific and Industrial Research Labs. Complex, Lahore (Pakistan). Food and Biotechnology Research Centre) | Zia-ur-Rehman (Pakistan Council of Scientific and Industrial Research Labs. Complex, Lahore (Pakistan). Food and Biotechnology Research Centre)
Factors affecting in vitro regeneration of shoots in shoot tip explant cultures of banana cultivar `Basrai', such as solid and liquid media, growth regulators, vitamins, and antioxidants were studied. Three-quarters strength of MS liquid medium supplemented with 17.75 muM 6-benzyladenine (BA), 11.42 muM indole-3-acetic acid (IAA) and 205 muM adenine sulphate induced the formation of mean number of 12.3 shoots, with the mean length of 3.0 cm, after three weeks of culture. Maximum shoot multiplication (14.33) occurred in liquid medium containing 22.19 muM BA. Addition of 2.0% activated charcoal (AC) to the liquid medium improved quality of the regenerated plants with expanded and glossy leaves, though the number of shoots was reduced (13.66). Profuse formation of roots was characteristically induced by AC. Addition of citric acid (CA) to the medium caused decline in morphogenetic expression of the cultures.
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