Evaluation of effectiveness BacT/ALERT system in monitoring microbiological quality and safety of food
2007
Sokolowska, B. | Niezgoda, J.,Instytut Biotechnologii Przemyslu Rolno-Spozywczego, Warszawa (Poland)
The purpose of the work was to evaluate usefulness of bioMerieux BacT/ALERT system as a tool to monitor microbiological quality of food products. Ten pasteurized or aseptic filled products - juices, beverages and soups - were examined. Samples, of 20 ml each, were contaminated with trial strains inoculum of Lactobacillus plantarum, Saccharomyces cerevisiae, Bacillus subtilis and Escherichia coli at two levels of contamination and set into BacT/ALERT and LYM or BacT/ALERT and AST bottles depending on the sample kind. The fastest to detect by BacT/ALERT system were Escherichia coli bacteria. Positive signal was observer just after 15 hours on AST medium with cucumber and traditional Polish soup made from fermented rye, with inoculum of 100 cfu/bottle. Time of detection of Lactobacillus plantarum strains in BacT/ALERT system was the longest one and ranged from 52.3 hours in tomato juice to 77.8 hours in red orange soft drink (inoculum of 26 cfu/bottle). Mash carrot juice and banana-carrot mash juice inhibited of Bacillus subtilis on LYM medium for 7 days, whereas the detection time of 38 cfu/bottle on AST medium ranged from 17 to 18 hours. B. subtilis and E. coli bacteria present in soups were to detect on AST medium after 15-18 hours, whereas on LYM medium this time extended even to 77.6 hours in sample with cucumber soup
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