Papaya ringspot virus (PRSV)-resistant GM papaya
2008
Anon.
Local papaya production suffered tremendously due to the ringspot virus. Thus, developing a virus-resistant variety is expected to make a big and lasting impact on the papaya industry. The application of genetic engineering to develop a ringspot resistant papaya offers the most promising approach to manage the virus. Magdalita and co-workers (2007) have been working on this area from July 1999 to the present. Findings of the study revealed the following: Thirty T2 plants were characterized agromorphologically. Selected plants from this batch were evaluated for PRSV resistance and presence of the coat protein (CP) gene. Remaining cultures of 138 putatively transformed somatic embryos are undergoing regeneration. A total of 130 isolates of PRSV was collected from all over the country for the determination of PRSV genetic diversity. Vector potential of aphids associated with papaya was evaluated from at least 300 individuals of each aphid species. Net sweeping of ground vegetation and vial tapping of papaya leaves showed that there were occasional insect visitors like spider mites, Negro bug, tree hopper, toy beetle, fungus gnats, silt-legged flies, pollinators (bees and skipper butterflies), beetles, wasps, and a number of predatory spiders (lynx spider, assassin spiders, linyphiids, and orb-weavers). The National Committee on Biosafety of the Philippines (NBCP) permit to conduct confined trial of the T3 generation of transgenic papaya lines at UPLB [University of the Philippines Los Banos, College, Laguna, Philippines] has been secured.
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