Chracterization of Verticillium dahliae isolates by fluorescent amplified fragment length polymorphisms (fAFLP)
2007
Abdelhalem, M.
Verticillium dahliae is a polyphagous plant fungus of high economic significance. A degree of genetic diversity exists among Verticillium dahliae isolates within and between vegetative compatibility groups (VCGs) that bears phytopathological significance and is worth investigating employing genetic tools of higher resolution than VCG. Due to their capacity to generate a large number of genome-wide genetic markers amplified fragment length polymorphisms (AFLPs) have been used in genetic, population and evolutionary studies of different fungal species. In the present study AFLPs was used to study genetic variation among 101 isolates of Verticillium dahliae from different VCGs (VCG 4B, 4AB and VCG 2A, 2B, 2AB), pathotype (tomato vs. eggplant), races (1 vs. 2), geographic origin and hosts. A dendrogram based on the AFLP data revealed four main clusters (A, B, C, D), in partial concordance to VCG subgroups. Analysis of molecular variance (AMOVA) demonstrated that isolates within a VCG subgroup are molecularly similar, to the extent that clustering of isolates correlated with VCG subgroups regardless of geographic origin and the host source. VCGs differed in molecular variability; the most variable ones being VCG4B and VCG4AB. Tomato isolates were grouped in four clusters clearly distinct from isolates derived from other plant hosts. Conversely, no significant molecular distinction was uncovered either between tomato and eggplant pathotypes or between race 1and 2.
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