Towards the authenticity of olive oil based on DNA chloroplast target and single nucleotide polymorphisms
2007
Khalil Awad, M.
Throughout the last decades, a number of methodologies are progressively being evolved at the molecular level to assist in discriminating Olea europea L. varieties. The study of the genetic relationships among olive Palestinian varieties and in relation to Greek varieties is very important in order to assess genetic diversity and structural details within a collection of sixty-one accessions, five Palestinian and fifty-six Greek, was analyzed using the sequences of two gene fragments and a panel of twenty-seven SNPs was identified. This group of single nucleotide polymorphisms is not efficient enough to discriminate among all the accessions. For example, two Palestinian cultivars were the same at all of the discovered polymorphic positions. However, the current data obtained for the Palestinian varieties can be used for the varietal survey and construction of a database of olive varieties grown in Palestine and to provide additional information that could constitute the basis for a rational scheme of breeding programs. In addition, the authenticity of olive oil which may have been adulterated with other plant species oils is another issue of great important. The fingerprinting of oil-producing plant species, taking into account the emphasis on olive oil authentication, was carried out using Experion lab-on-a-chip capillary electrophoresis. The application of this technique was carried out by amplifying the chloroplastic trnL (UAA) intron within a collection of ten species. The discrimination among them is based on the length of chloroplastic PCR amplicons. In spite of that, this technique was not completely sufficient to identify all of them because of its restricted resolution.
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