Phenolic prophile and antioxidant activity of the date palm (Phoenix dactylifera) seeds
2007
Messaoudi, R.
The total phenolic content of methanol and ethyl-acetate extract of seeds from seven Algerian varieties of ripe date palm fruits (phoenix dactylifera) was estimated by the Folin-Ciocalteu method. Their respective antioxidant activities were investigated by two in-vitro methods, the DPPH and the chemiluminescence method and their phenolic profile was determined by LC-DAD-MS (ESI- and ESI+). The total polyphenol content ranged between 27.22 and 38.50 mg/100 g fresh weight (FW) for the methanol extracts and between 22.80 and 42.65 mg/100 g FW for the ethyl acetate extracts. The different extracts showed an antioxidant activity with the two tests, giving values of antiradical efficiency from 0.15±0.01 to 0.33±0.02 and from 0.04±0.02 to 0.15±0.04 for the methanol and the ethyl-acetate extracts, respectively, and of scavenging activity from 1.30±0.52 to 3.46±0.23 and from 0.43±0.01 to 1.58±0.58 for the methanol and the ethyl-acetate extracts, respectively. The correlation among these tests was rather weak to medium. All the varieties were found to contain mainly catechin or epi catechin derivative, sinapic, cinnamic, and coummaric acid derivatives. Different flavonoids were identified and could serve as a tool for the variety discrimination; the most relevant compound was the glycosylated aurone from Tazizaout variety. Further investigation of this variety for isolation, structural determination and purification of this secondary metabolite would be of interest.
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