Influence of bioactive compounds extracted from mushroom Ganoderma lucidum on B and T cells
2009
Klaus, A.S., University of Belgrade, Belgrade - Zemun (Serbia). Faculty of Agriculture, Institute for Food Technology | Kozarski, M.S., University of Belgrade, Belgrade - Zemun (Serbia). Faculty of Agriculture, Institute for Food Technology | Niksic, M.P., University of Belgrade, Belgrade - Zemun (Serbia). Faculty of Agriculture, Institute for Food Technology
The aim of this experiment was to investigate the effects of different concentrations of isolated Ganoderma lucidum GL-1 extract on the growth of JY (B) and Jurkat (T) cells. Obtained extracts were added to the cells in concentrations 1 mg/ml, 100 ng/ml, 10 ng/ml, 1 ng/ml and 100 pg/ml. JY and Jurkat cells were exposed to the action of bioactive compounds, b-glucans, during the incubation period of 72 h, at 37 deg C, on the atmosphere with 5% CO2 and their number was counted. Among all tested concentrations of extract the most important influence showed concentration of 1mg/ml, which reduced the number of B cells by 61.46%, while in the case of T cells, their number was reduced by 57.14%.
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