Induction of somatic embryogenesis in Pinus heldreichii culture
2007
Stojicic, D., Institute of Forestry, Belgrade (Serbia) | Uzelac, B., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia) | Janosevic, D., University of Belgrade, Belgrade (Serbia). Faculty of Biology, Institute of Botany | Culafic, Lj., University of Belgrade, Belgrade (Serbia). Faculty of Biology, Institute of Botany | Budimir, S., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia)
The potential for somatic embryogenesis in zygotic embryo and megagametophyte culture of Pinus heldreichii was examined. Somatic embryogenesis was initiated from megagametophytes containing immature zygotic embryos at early stages of development. An induction frequency of up to 6.7% was obtained on Gresshoff and Doy medium in the presence of 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/l benzyladenine (BA). Formation and further proliferation of embryogenic tissue were achieved upon transfer of explants to a medium with reduced levels of growth regulators. Somatic embryos are being cultured for further development.
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