Microbiological and mxcotoxicological examintions of grains grown in Slovenia | Mikrobioloske in mikotoksikoloske preiskave zitaric v Sloveniji
2008
Jakovac-Strajn, B, Ljubljana Univ. (Slovenia). Veterinary Fac. | Vengust, A. | Pestevsek, U. | Tavcer-Kalcher, G. | Ujcic-Vrhovnik, I. | Pavsic-Vrtac, K.
The research was aimed to determine the contamination of grains, cultivated and used for animal diets by Slovenian farmers. The total of 101 samples of several different grains (corn, barley, wheat, triticale, oat, fooder pea) were examined on saprophytic microorganisms. The largest average number of colony forming units (cfu) of bacteria was found in barley (2610x103/g), whereas Fusarium, Penicillium and Mucor (250, 744 in 5 x 103 cfu/g) were detected mostly in corn. The leading contaminants in oat, fooder pea and triticale were mostly moulds from genus Aspergillus (205x103 cfu/g). The contamination of samples with yeasts varied from 2 to 11x103 cfu/g. Liquid (HPLC) and gas chromatography (GC) methods proved the concentrations of various mycotoxins in 73% of all samples; mostly with DON (66%) in concentrations of 0.150-14.420 mg/kg, followed by ZON (30%, 20-630 microg/kg), fumonisin B1 (28%, 10-4863 microg/kg), fumonisin B2 (21%, 10-1629 microg/kg), while HT-2 and T-2 toxins were found in low concentrations in two samples, but aflatoxin B1 and ochratoxin A in one sample. The results are pointing out that further control of saprophytic microorganisms and mycotoxins in Slovenian primary grain production is thus required and justified.
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