Wheat genomics: Challenge for the 21st century | Genomika pšenice: Výzva pro 21. stoleti
2008
Šafář, J., Ústav experimentální botaniky AV ČR, Olomouc (Czech Republic) | Šimková, H., Ústav experimentální botaniky AV ČR, Olomouc (Czech Republic) | Kubalákova, M., Ústav experimentální botaniky AV ČR, Olomouc (Czech Republic) | Bartoš, J., Ústav experimentální botaniky AV ČR, Olomouc (Czech Republic) | Doležel, J., Ústav experimentální botaniky AV ČR, Olomouc (Czech Republic)
The bread wheat is unique among the important agricultural crops in possessing massive genome with a prevalence of dispersed DNA repeats. Polyploidy and the presence of repeats make gene cloning and genome sequencing in wheat extremely difficult. Chromosome genomics simplifies these tasks by targeting single chromosomes and chromosome arms, which represent only a few percent of the nuclear genome. The advantages of this strategy over a whole-genome approach include the avoidance of problems due to the presence of homoeologs, reduction of work to manageable portions, cost efficiency, and an opportunity to structure collaborative projects where individual laboratories work on particular chromosomes. We discuss various applications of flow-sorted chromosomes and assess the potential of chromosome genomics in the Triticeae.
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