The effect of antiserum to heat-shock proteins 70 (HSP 70) on the in vitro development of porcine embryos exposed to hyperthermia
2008
Olexiková, L., Slovak University of Agriculture in Nitra (Slovak Republic). Faculty of Agrobiology and Food Resources. Department of Environmental Sciences and Zoology | Makarevich, A.V., Slovak Centre of Agricultural Research, Nitra (Slovak Republic) | Kubovičová, E., Slovak Centre of Agricultural Research, Nitra (Slovak Republic) | Pivko, J., Slovak Centre of Agricultural Research, Nitra (Slovak Republic)
We studied the influence of short-term exposure (6 h) to the elevated temperature (41.5 degree C) on the vitality of porcine embryos with blocked heat-shock proteins 70 (Hsp70). Intrinsic Hsp70 in embryos was blocked by adding the antiserum to Hsp70. The embryos at morula stage were cultured either at standard temperature (37.5 degree C) or hyperthermic (41.5 degree C) conditions for 6h and post-incubated at 37.5 degree C for 24 h. Embryo development to the blastocyst stage, embryo cell number and apoptosis occurrence were examined. Hyperthermia alone did not affect the blastocyst rate, cell number or apoptosis (TUNEL-index) compared to control. However, anti-Hsp70 addition caused significant decreases in cell number and blastocyst rate, whilst TUNEL-index was dramatically increased compared to non-blocked embryos. These results show that brief exposure to hyperthermia does not affect embryo development, whereas the blockage of Hsp70 leads to developmental failure mainly under hyperthermic conditions.
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par Agricultural Knowledge and Innovation Institute
Découvrez la collection de ce fournisseur de données dans AGRIS