In vitro efficacy of the antifungal activity of some Thai medicinal-plants on the pathogenic fungus, Saprolegnia parasitica H2, in fish | เธเธฃเธฐเธชเธดเธเธเธดเธเธฅเธเธญเธเธชเธกเธธเธเนเธเธฃเนเธเธขเธเธฒเธเธเธเธดเธเธเนเธญเธเธฒเธฃเธขเธฑเธเธขเธฑเนเธเธเธฒเธฃเนเธเธฃเธดเธเนเธเธดเธเนเธเธเธญเธเนเธเธทเนเธญเธฃเธฒเธเธตเนเธเนเธญเนเธฃเธเนเธเธเธฅเธฒเนเธเธซเนเธญเธเธเธเธดเธเธฑเธเธดเธเธฒเธฃ
2007
Pareeya Udomkusonsri(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Veterinary Medicine. Department of Pharmacology) E-mail:[email protected] | Kamolchai Trongvanichnam(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Veterinary Medicine. Department of Pharmacology) | Malinee Limpoka(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Veterinary Medicine. Department of Pharmacology) | Narumol Klangkaew(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Veterinary Medicine. Department of Pharmacology) | Napasorn Kusucharit(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Veterinary Medicine. Department of Pharmacology)
Ethanol crude extracts of galanga (Alpinia galanga) (rhizome), betel vine (Piper betel Linn.) (leaves), Rhinacanthus nasutus Linn. (leaves), Kaempferia galanga Linn. (leaves and roots) were studied for fungistatic and fungicidal activity against water mold Saprolignea parasitica H2. The crude extracts were dissolved in water and mixed in glucose yeast extract agar at the concentrations of 5-500 micro g/ml. It was found that MIC (minimal inhibitory concentration) of crude extracts of betel vine and K. galanga Linn. were 500 and 125 micro g/ml, respectively. The crude extracts of galanga, R. nasutus Linn., and K. galanga at 500 micro g/ml had minimal fungistatic activity against S. parasitica H2. The fungicidal concentrations of crude extracts of betel vine, R. nasutus Linn. and K. galanga Linn. against S. parasitica H2 were 500, 5000 and 50 micro g/ml, respectively. Crude extracts of galangal and K. galanga at 5000 micro g/ml had no fungicidal effects in this study. In addition, the fungicidal activity varied between concentration of plant extract and exposure time. This study presented that the crude extracts from betel vine and K. galanga Linn. had high antifungal activity against S. parasitica H2, while and R. nasutus Linn. leaves had moderate activity.
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