Development of TaqMan real-time reverse transcription-polymerase chain reaction for the quantification of Feline Leukemia Virus viral load | การพัฒนาปฏิกิริยา TaqMan real-time reverse transcription-polymerase chain reaction เพื่อการตรวจหาปริมาณเชื้อ Feline Leukemia Virus
2009
Witchaya Tongtako(Kasetsart University, Bangkok (Thailand). Faculty of Veterinary Medicine. Department of Pathology) | Theerapol Sirinarumitr(Kasetsart University, Bangkok (Thailand). Faculty of Veterinary Medicine. Department of Pathology)
Feline leukemia virus (FeLV), a gamma retrovirus of domestic cat, is not only veterinary interest but also it is considered to be an importance model for study pathogenesis of tumor and acquired immunodeficiency syndrome (AIDS) in many animals. After initial infection, disease progression has been associated with cellular and humoral immune response and FeLV proviral load. Moreover, the recent study showed that not only the proviral load but also the plasma viral loads are important parameters which associates the disease progression. In this study, we developed the TaqMan real-time reverse transcription-polymerase chain reaction (RT-PCR) for the quantification of FeLV viral loads. The assay was developed to amplify conserve fragment within unique region (U3) of long terminal repeat (LTR). The detection limit of this assay was 8.3 copies of RNA standard template or 4.15 viruses per reaction. Thus, 1,778 viruses per milliliters of plasma or serum can be detected.
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