Screening of soil antagonistic bacteria for watermelon fusarium wilt
2008
Hu Xueqin | Zhang Hongbin | Su Xiaofei
[Objective] The aim of this study was to screen bacteria strains with stable antagonistic effect against watermelon fusarium wilt from soil and investigate the biological control of watermelon fusarium wilt by applying the antagonistic bacteria strains into soil. [Method] Actinomycete strains, fluorescent bacteria strains and bacillus strains were isolated from soil samples by the dilution-plate method, then its resistance was screened respectively by the improved confront culture method after colonies were purified. Finally, bacteria strains with better antagonistic effect were identified. [Result] 29 bacteria strains with stable antagonistic effect against watermelon fusarium wilt were screened from 39 soil samples, which contained 15 fluorescent bacteria strains, 5 bacillus strains and 9 actinomycete strains. Furthermore, three antagonistic bacteria strains of FM2, FM3 and FM4 with the strongest antagonism were identified primarily. [Conclusion] According to cultural characteristics, morphological observation, biochemical and physiological tests, FM2 belongs to bacillus subtilis, while FM3 and FM4 belong to micrococcus.
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