Isozyme phenotypic difference of Varicorhinus macrolepis malic enzyme | 泰山赤鳞鱼苹果酸酶同工酶表型差异的研究
2009
Pang Qiuxiang, Shandong University of Technology, Zibo (China), School of Life Sciences | Pang Shuxiang, Biology andChemistry Department of Jilin College, Jilin (China) | Zhao Bosheng, Shandong University of Technology, Zibo (China), School of Life Sciences
By using polyacrylamide gel electrophoresis and biochemical straining method, malic enzyme isozyme in 10 organs of male or female Varicorhinus macrolepis were analyzed. The results showed that malic enzyme exists in heart, gonads, eyes and gills tissues in both female and male V.macrolepis. There was only one ME-2 in heart and ME-1 in eye of female fish, while there were ME-1 and ME-2 in heart and ME-2 in eyes of male fish. Ovary contained ME-1 and ME-2, while spermary only contained ME-1. In kidney and spleen tissues, malic enzyme was found only in female fish, while in muscle, malic enzyme existed only in male fish. The phenotypic of male enzyme had obvious differences no matter in the same organ of different sexes in V.macrolepis or different organs in the same sex. But there were no differences among different individuals of the same sex. The results will provide basic data for developmental genetics, breed improvement and directed breeding of V.macrolepis, and lay a foundation for its development and protection.
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