Optimizing Yanbian cow oocytes mature in vitro and cleavage system after nuclear transfer based on uniform design | 基于均匀设计优化延边黄牛卵母细胞体外成熟及核移植后卵裂体系
2009
Miao Tingting, .Tonghua Normal University, Tonghua (China), Department of Biology | Wei Shibao, Animal Health Supervision in Tonghua Country, Tonghua (China)
[Objective] The aim was to optimize Yanbian cow oocytes mature in vitro and cleavage system after nuclear transfer based on uniform design. [Method] Oocytes were recovered by aspiration method, and oocytes were matured in vitro (IVM) with different conditions, and then carried out nucleus transfer, fusion, activation and in vitro culture (IVC) of embryo. Effects of ovary storage temperature, maturation time and follicular diameter size on in vitro maturation and cleavage rates of cow oocytes were compared. [Result] The best conditions of IVM of Yanbian cow oocytes was that: the oocytes of 8 mm diameter were matured in vitro for 24 hours when the ovaries were stored at 26 ℃ or 31 ℃. The best cleave conditions after nucleus transfer of oocytes was that: the oocytes of 6 mm or 8 mm diameter were cultured in vitro for 24 hours when the ovaries were stored at 26 ℃. [Conclusion] The result has some reference to Yanbian cow and other cow breeding and population expanding propagation.
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