Callus induction from mature embryos of maize | 玉米成熟胚愈伤组织的诱导
2009
Xiao LiJie, Heilongjiang August First Land Reclamation University, Daqing (China), College of Life Science and Technology | Wang Liyan, Heilongjiang August First Land Reclamation University, Daqing (China), College of Life Science and Technology | Min Li, Crop Research and Breeding Center of Land Reclamation
In this study we studied the factors influencing the callus induction from mature embryos of maize inbred lines Qi 319, Zhen 58, Chang 7-2, Lx 9801 and 81162, such as genotype, combination of plant growth regulators, and low-temperature pretreatment. The results showed that the induction rate of Qi 319 was the highest among the four genotypes tested; combination of 4.0 mg/L 2,4-D + 0.5 mg/L 6-BA was suitable for inducing callus from mature embryos; three days of 4℃ pretreatment can promote the callus induction significantly. The indices optimized in the present study are helpful for establishing genetic transformation system in maize without considering seasonal variation.
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