Analysis of mannitol, as tracer of bacterial infections in cane and beet sugar factories
2009
Eggleston, G.,USDA-ARS-Southern Regional Research Center, New Orleans (USA)
Mannitol, formed mainly by Leuconostoc mesenteroides bacteria, is a sensitive marker of sugarcane and sugar beet deterioration that can predict multiple processing problems. An enzymatic factory method that is rapid, simple, and inexpensive is now available to measure mannitol in consignment juices at both sugarcane and sugar beet factories. Mannitol is directly measured on a spectrophotometer using mannitol dehydrogenase as the enzyme. The stability of the reagents, limited juice preparation, and linearity are described. The method is accurate compared to ion chromatography and precise, although precision decreases for both sugarcane and sugarbeet juices with low mannitol concentrations. A strong polynomial relationship (R2 = 0.912) existed between mannitol and haze dextran in juices obtained across a 3-month processing season at a sugarcane factory. Mannitol concentrations are usually higher than concentrations of monoclonal antibody dextran and other specific measures of dextran, which indicates (I) the usefulness and sensitivity of mannitol to predict sugarcane deterioration from Leuconostoc and other bacteria, and (II) the underestimation by sugar industry personnel of the relatively large amounts of mannitol present in deteriorated sugarcane. Greater than 250-500 ppm/Brix of mannitol in sugarcane juice predicts downstream processing problems, but this threshold value may vary from region to region. The increasing awareness of how mannitol detrimentally effects processing, e.g., its contribution to the Louisiana hard-to-boil massecuite phenomenon, is also fully discussed.
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