Effect of the addition of insulin-transferrin-selenium and ascorbic acid in the in vitro maturation medium of prepubertal bovine oocytes | Efecto de la adición de insulina-transferrina-selenio y ácido ascórbico en el medio de maduración in vitro de ovocitos procedentes de terneras prepúberes
2009
Córdova, B.L., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària | Morató, R., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària | Izquierdo, M.D., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària | Paramio, M.T., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària | Mogas, T., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària
The purpose of this study was to evaluate the effects of the addition of insulin-transferrin-selenium (ITS) and ascorbic acid to the in vitro maturation medium on the chromosome configuration, cortical granules (CG) distribution and further embryo development of prepubertal bovine oocytes. Immature calf oocytes were randomly assigned to three experimental groups: (1) TCM: TCM 199 with 10% fetal bovine serum (FBS) and 10 micro g/ml Epidermal Growth Factor (EGF); (2) TCM+ITS: TCM199 with 10% FBS, 10 micro g/ml EGF and 5 micro g/ml ITS; (3) TCM+ITS+ASC: TCM199 with 10% FBS, 10 micro g/ml EGF and 5 micro g/ml ITS and 10 micro g/ml ascorbic acid. After in vitro maturation, samples of calf oocytes from each experimental group were fixed and evaluated after in vitro maturation using specific fluorescent probes. the remaining oocytes were fertilized and cultured in vitro. Cleavage and blastocyst rates were determined on days 2 and 8 after fertilization, respectively. CG and chromosome distribution did not differ between experimental groups as well as cleavage rate at 48 hpi. However, supplementation with ITS and acid ascorbic or just ITS yielded significantly lower blastocyst rates than TCM group. These results indicate that the supplementation of the IVM medium with ITS with or without ascorbic acid does not improve the subsequent embryo development of prepubertal bovine oocytes.
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