IMPROVEMENT OF INCUBATION CONDITIONS, BUFFER SYSTEM AND THE EFFECT OF AIRFLOW ON KINETIC GROWTH RATE OF THEILERIA ANNULATA SCHIZONT INFECTED BOVINE MYELOID CELL
2004
Hosseini Vafa , N., Razi Vaccine and Serum Research Institute | Esmaeil-Nia, K., Razi Vaccine and Serum Research Institute | Abdigoudarzi, M., Razi Vaccine and Serum Research Institute | Hashemi Fesharki, R., Razi Vaccine and Serum Research Institute
Kinetic growth rate of the Theileria annulata (T.annualata) schizont infected monocyte-macrophage cell (SIMC) using modified Stoker medium in different incubation conditions was studied. In this regard 28 Roux flasks were divided into four groups each of 7. Two groups (one with HEPES and the other without HEPES) were incubated with carbon dioxide flow. The reminder two groups were incubated under conventional condition. All bottles were observed for viability test and pH changes daily up to 7 days. To study the effect of airflow on cell growth rate some glass tubes with different diameter were inserted through stopper of Roux bottles containing cultured SIMC. The results indicate that carbon dioxide flow together with HEPES have an increasing effect on cell quantity moreover, the specific growth rate index of all groups were similar. The different amounts of airflow doesn't have any effect on oxygen consumption, therefore the routine culture method using the trapped air in bottles, is the most suitable method for making a suspension culture of T.annulata SIMCs.
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